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    Monobind free t3 ft3 levels
    Free T3 Ft3 Levels, supplied by Monobind, used in various techniques. Bioz Stars score: 96/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/free+t3/Free+T3+AccuLite+CLIA+Kits/pm41856004-117-8-12
    Average 96 stars, based on 2 article reviews
    free t3 ft3 levels - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Supplementation of soybean meal and canola meal with ethylenediamine dihydroiodide and Ascophyllum nodosum on iodine metabolism, production performance, and nutrient utilization in dairy cows.
    Article Snippet: After collection, tubes were transported to the laboratory and centrifuged (Eppendorf model 5810) at 1,278 × g (20 min, 4°C). .. The time elapsed between blood sample collection and centrifugation took ~90 min. Serum samples (1 mL) were pipetted into cryovials and stored at −80°C until analyzed for BUN (kit #0580; Stanbio Laboratory), total triiodothyronine (T3; kit #8025–300), total thyroxine (T4; kit #8025–300), free T3 (kit #7025–300), free T4 (kit # 7025–300), and thyroid-stimulating hormone (TSH; kit #8025–300), with all thyroid hormone kits purchased from Monobind, Inc. Thyroid hormones were analyzed using a chromate microplate reader (Awareness Technology, Inc.) set at a wavelength of 450 nm, and BUN analyzed colorimetrically with a UV/Vis spectrophotometer (Beckman Coulter Inc.) set at 520 nm wavelength. .. Serum samples (200 μL) for I analysis were also stored in cryovials at −80°C until shipped to the Michigan State University Veterinary Diagnostic Laboratory.

    Spectrophotometry:

    Article Title: Supplementation of soybean meal and canola meal with ethylenediamine dihydroiodide and Ascophyllum nodosum on iodine metabolism, production performance, and nutrient utilization in dairy cows.
    Article Snippet: After collection, tubes were transported to the laboratory and centrifuged (Eppendorf model 5810) at 1,278 × g (20 min, 4°C). .. The time elapsed between blood sample collection and centrifugation took ~90 min. Serum samples (1 mL) were pipetted into cryovials and stored at −80°C until analyzed for BUN (kit #0580; Stanbio Laboratory), total triiodothyronine (T3; kit #8025–300), total thyroxine (T4; kit #8025–300), free T3 (kit #7025–300), free T4 (kit # 7025–300), and thyroid-stimulating hormone (TSH; kit #8025–300), with all thyroid hormone kits purchased from Monobind, Inc. Thyroid hormones were analyzed using a chromate microplate reader (Awareness Technology, Inc.) set at a wavelength of 450 nm, and BUN analyzed colorimetrically with a UV/Vis spectrophotometer (Beckman Coulter Inc.) set at 520 nm wavelength. .. Serum samples (200 μL) for I analysis were also stored in cryovials at −80°C until shipped to the Michigan State University Veterinary Diagnostic Laboratory.

    Article Title: Prepartum supplementation of Ascophyllum nodosum meal or ethylenediamine dihydroiodide: Effects on colostrum yield and growth and health of dairy calves.
    Article Snippet: All other blood samples were centrifuged at 1,278 × g at 4°C for 20 min. Serum collected at 0 h and 24 h from tubes without additive was frozen at −20°C and shipped to the Saskatoon Colostrum Co. Ltd. for IgG analysis using a radial immunodiffusion assay. .. Plasma collected from EDTA tubes was frozen at −20°C and later analyzed inhouse for total T3 (kit #125–300; inter-assay CV = 8.60%, intra-assay CV = 8.86%), total thyroxin (T4; kit #225–300; interassay CV = 3.86%, intra-assay CV = 5.62%), free T3 (kit #1325–300; inter-assay CV = 8.32%, intra-assay CV = 7.01%), and free T4 (kit #1225–300; inter-assay CV = 7.48%, intra-assay CV = 7.63%) using ELISA commercial kits (Monobind Inc.) with an Epoch microplate spectrophotometer equipped with the Gen5 analysis software (Agilent) and absorbances read at 450 nm wavelength. ..

    Clinical Proteomics:

    Article Title: Prepartum supplementation of Ascophyllum nodosum meal or ethylenediamine dihydroiodide: Effects on colostrum yield and growth and health of dairy calves.
    Article Snippet: All other blood samples were centrifuged at 1,278 × g at 4°C for 20 min. Serum collected at 0 h and 24 h from tubes without additive was frozen at −20°C and shipped to the Saskatoon Colostrum Co. Ltd. for IgG analysis using a radial immunodiffusion assay. .. Plasma collected from EDTA tubes was frozen at −20°C and later analyzed inhouse for total T3 (kit #125–300; inter-assay CV = 8.60%, intra-assay CV = 8.86%), total thyroxin (T4; kit #225–300; interassay CV = 3.86%, intra-assay CV = 5.62%), free T3 (kit #1325–300; inter-assay CV = 8.32%, intra-assay CV = 7.01%), and free T4 (kit #1225–300; inter-assay CV = 7.48%, intra-assay CV = 7.63%) using ELISA commercial kits (Monobind Inc.) with an Epoch microplate spectrophotometer equipped with the Gen5 analysis software (Agilent) and absorbances read at 450 nm wavelength. ..

    Article Title: Thyroid hormone profile during annual reproductive cycle of diploid and triploid catfish, Heteropneustes fossilis (Bloch).
    Article Snippet: Triploid Wshes generally show sterility along with retarded gonadal development and aneuploid gametes.. In teleosts, thyroid hormones inXuence seasonal adaptations and annual events such as reproduction.. In addition, thyroid hormone deposition in matured ova is important for reproductive success as the role of thyroid hormones in early development and metamorphosis is well established.

    Inter Assay:

    Article Title: Prepartum supplementation of Ascophyllum nodosum meal or ethylenediamine dihydroiodide: Effects on colostrum yield and growth and health of dairy calves.
    Article Snippet: All other blood samples were centrifuged at 1,278 × g at 4°C for 20 min. Serum collected at 0 h and 24 h from tubes without additive was frozen at −20°C and shipped to the Saskatoon Colostrum Co. Ltd. for IgG analysis using a radial immunodiffusion assay. .. Plasma collected from EDTA tubes was frozen at −20°C and later analyzed inhouse for total T3 (kit #125–300; inter-assay CV = 8.60%, intra-assay CV = 8.86%), total thyroxin (T4; kit #225–300; interassay CV = 3.86%, intra-assay CV = 5.62%), free T3 (kit #1325–300; inter-assay CV = 8.32%, intra-assay CV = 7.01%), and free T4 (kit #1225–300; inter-assay CV = 7.48%, intra-assay CV = 7.63%) using ELISA commercial kits (Monobind Inc.) with an Epoch microplate spectrophotometer equipped with the Gen5 analysis software (Agilent) and absorbances read at 450 nm wavelength. ..

    Intra Assay:

    Article Title: Prepartum supplementation of Ascophyllum nodosum meal or ethylenediamine dihydroiodide: Effects on colostrum yield and growth and health of dairy calves.
    Article Snippet: All other blood samples were centrifuged at 1,278 × g at 4°C for 20 min. Serum collected at 0 h and 24 h from tubes without additive was frozen at −20°C and shipped to the Saskatoon Colostrum Co. Ltd. for IgG analysis using a radial immunodiffusion assay. .. Plasma collected from EDTA tubes was frozen at −20°C and later analyzed inhouse for total T3 (kit #125–300; inter-assay CV = 8.60%, intra-assay CV = 8.86%), total thyroxin (T4; kit #225–300; interassay CV = 3.86%, intra-assay CV = 5.62%), free T3 (kit #1325–300; inter-assay CV = 8.32%, intra-assay CV = 7.01%), and free T4 (kit #1225–300; inter-assay CV = 7.48%, intra-assay CV = 7.63%) using ELISA commercial kits (Monobind Inc.) with an Epoch microplate spectrophotometer equipped with the Gen5 analysis software (Agilent) and absorbances read at 450 nm wavelength. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Prepartum supplementation of Ascophyllum nodosum meal or ethylenediamine dihydroiodide: Effects on colostrum yield and growth and health of dairy calves.
    Article Snippet: All other blood samples were centrifuged at 1,278 × g at 4°C for 20 min. Serum collected at 0 h and 24 h from tubes without additive was frozen at −20°C and shipped to the Saskatoon Colostrum Co. Ltd. for IgG analysis using a radial immunodiffusion assay. .. Plasma collected from EDTA tubes was frozen at −20°C and later analyzed inhouse for total T3 (kit #125–300; inter-assay CV = 8.60%, intra-assay CV = 8.86%), total thyroxin (T4; kit #225–300; interassay CV = 3.86%, intra-assay CV = 5.62%), free T3 (kit #1325–300; inter-assay CV = 8.32%, intra-assay CV = 7.01%), and free T4 (kit #1225–300; inter-assay CV = 7.48%, intra-assay CV = 7.63%) using ELISA commercial kits (Monobind Inc.) with an Epoch microplate spectrophotometer equipped with the Gen5 analysis software (Agilent) and absorbances read at 450 nm wavelength. ..

    Article Title: Association between TPO Asn698Thr and Thr725Pro gene polymorphisms and serum anti-TPO levels in Iranian patients with subclinical hypothyroidism.
    Article Snippet: .. The Free T3, Free T4, TSH and thyroid peroxidase antibody levels were measured via the Enzyme-linked immune absorbent assay (ELISA) method (Monobind, USA) using a plate reader (BioTek, USA). .. For analysis of gene polymorphisms, blood samples were collected in EDTA-coated tubes and stored at -20 °C until genomic DNA was extracted using the Proteinase K column method according to the protocol of the manufacturer (Sinaclon, Iran).

    Article Title: Asprosin is a centrally-acting orexigenic hormone
    Article Snippet: Human adiponectin, mouse adiponectin, human leptin, mouse leptin, human ghrelin (total) and mouse ghrelin (total) ELISA kits (Millipore, Billerica, MA) were used to determine the concentrations of each plasma parameter in flash-frozen, previously unthawed plasma as per the manufacturer’s instructions. .. TSH, free T3, and free T4 were measured using an ELISA kit (Monobind Inc, CA) as per the instructions provided. ..

    Article Title: Thyroid hormone profile during annual reproductive cycle of diploid and triploid catfish, Heteropneustes fossilis (Bloch).
    Article Snippet: Triploid Wshes generally show sterility along with retarded gonadal development and aneuploid gametes.. In teleosts, thyroid hormones inXuence seasonal adaptations and annual events such as reproduction.. In addition, thyroid hormone deposition in matured ova is important for reproductive success as the role of thyroid hormones in early development and metamorphosis is well established.

    Article Title: Asprosin is a centrally acting orexigenic hormone.
    Article Snippet: Human adiponectin, mouse adiponectin, human leptin, mouse leptin, human ghrelin (total) and mouse ghrelin (total) ELISA kits (Millipore, Billerica, MA) were used to determine the concentrations of each hormone in flash-frozen, previously unthawed plasma as per the manufacturer’s instructions. .. TSH, free T3, and free T4 were measured using an ELISA kit (Monobind Inc., CA) as per the instructions provided. ..

    Article Title: Preventive effects of Aframomum melegueta extracts on the reproductive complications of propylthiouracil-induced hypothyroidism in male rat.
    Article Snippet: Infertility is a disease of the reproductive system defined by the fail‐ ure to achieve a clinical pregnancy after 12 months of regular and unprotected sexual intercourse (Vander‐Borght & Wyns, 2018).. It is estimated to affect between 8% and 12% of reproductive‐aged couples worldwide (Vander‐Borght & Wyns, 2018).. Male factors account for 20%–30% of infertility cases (Vander‐Borght & Wyns, 2018).

    Article Title: Age-Related Features of the Function of the Hypothalamic-Pituitary-Thyroid (HPT) Axis in Nonhuman Primates under Constant Lighting.
    Article Snippet: We studied the features of the functioning of the hypothalamic—pituitary—thyroid (HPT) axis under conditions of constant (4 weeks) lighting (LED lamps intended for office and residential premise) on a translational model of young adult and old female rhesus monkeys, in particular taking into account their behavior.. Constant lightning had no significant effect on the levels of thyroid stimulating hormone, thyroxine, and triiodothyronine under basal conditions in all animals, regardless of age and behavioral characteristics, but induced a decrease in thyroid function under conditions of its activation with thyrotropin releasing hormone, mainly in old animals.

    Software:

    Article Title: Prepartum supplementation of Ascophyllum nodosum meal or ethylenediamine dihydroiodide: Effects on colostrum yield and growth and health of dairy calves.
    Article Snippet: All other blood samples were centrifuged at 1,278 × g at 4°C for 20 min. Serum collected at 0 h and 24 h from tubes without additive was frozen at −20°C and shipped to the Saskatoon Colostrum Co. Ltd. for IgG analysis using a radial immunodiffusion assay. .. Plasma collected from EDTA tubes was frozen at −20°C and later analyzed inhouse for total T3 (kit #125–300; inter-assay CV = 8.60%, intra-assay CV = 8.86%), total thyroxin (T4; kit #225–300; interassay CV = 3.86%, intra-assay CV = 5.62%), free T3 (kit #1325–300; inter-assay CV = 8.32%, intra-assay CV = 7.01%), and free T4 (kit #1225–300; inter-assay CV = 7.48%, intra-assay CV = 7.63%) using ELISA commercial kits (Monobind Inc.) with an Epoch microplate spectrophotometer equipped with the Gen5 analysis software (Agilent) and absorbances read at 450 nm wavelength. ..



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    Thyroid status of the study patients at the time of the thyroid surgery. (A) Two patients with Graves’ disease on methimazole who also took Lugol's solution for 10 days before the surgery had thyroid function tests in the overt hypothyroid range at the time of the surgery (TSH 18.17 µ IU/L [reference range 0.4-4.5 µIU/L], free T4 0.6 ng/dL [reference range 0.8-1.8 ng/dL], <t>free</t> <t>T3</t> 3.0 pg/mL [reference range 2.3-4.2 pg/mL]; TSH 18.48 µIU/L, free T4 0.4 ng/dL, free T3 2.2 pg/mL), respectively; 1 patient was hypothyroid for 5 weeks, whereas the second patient was euthyroid/hypothyroid for 4 weeks while taking methimazole before starting Lugol's solution before the thyroid surgery. (B) One patient with a hyperfunctioning thyroid nodule/Graves’ disease who was on methimazole and took Lugol's solution for 10 days before the surgery had thyroid function tests within normal range at the time of the surgery (TSH 0.77 µIU/L, free T4 0.8 ng/dL, free T3 2.8 pg/mL); the patient was euthyroid on methimazole for at least 2 months before surgery. (C) One euthyroid patient with benign thyroid nodules had only subcutaneous adipose tissue (no deep adipose tissue) removed during the surgery. (D) One patient who underwent thyroid surgery for thyroid cancer had a minimally elevated TSH of 4.4 µIU/L before the surgery (reference range 0.36-3.7 µIU/L), not on thyroid hormone treatment; because all prior TSH levels were normal, this patient was included in the euthyroid patient group. (E) One patient with an untreated hyperfunctioning thyroid nodule had thyroid function tests in the subclinical hyperthyroid range for at least 1.5 years before surgery; TSH 0.07 µIU/L (reference range 0.4-4.5 µIU/L), free T4 1.2 ng/dL (reference range 0.8-1.8 ng/dL), free T3 3.9 pg/mL (reference range 2.3-4.2 pg/mL) at surgery time. (F) One patient with Graves’ disease took methimazole in the past for only 1 month and then stopped taking this medication 6 months before thyroid surgery; the patient also did not take Lugol's solution, as prescribed before the surgery. Thyroid function tests showed overt hyperthyroidism at the surgery time (TSH 0.02 µ IU/L [reference range 0.4-4.5 µIU/L], free T4 3.4 ng/dL [reference range 0.93-1.7 ng/dL]).
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    Thyroid status of the study patients at the time of the thyroid surgery. (A) Two patients with Graves’ disease on methimazole who also took Lugol's solution for 10 days before the surgery had thyroid function tests in the overt hypothyroid range at the time of the surgery (TSH 18.17 µ IU/L [reference range 0.4-4.5 µIU/L], free T4 0.6 ng/dL [reference range 0.8-1.8 ng/dL], <t>free</t> <t>T3</t> 3.0 pg/mL [reference range 2.3-4.2 pg/mL]; TSH 18.48 µIU/L, free T4 0.4 ng/dL, free T3 2.2 pg/mL), respectively; 1 patient was hypothyroid for 5 weeks, whereas the second patient was euthyroid/hypothyroid for 4 weeks while taking methimazole before starting Lugol's solution before the thyroid surgery. (B) One patient with a hyperfunctioning thyroid nodule/Graves’ disease who was on methimazole and took Lugol's solution for 10 days before the surgery had thyroid function tests within normal range at the time of the surgery (TSH 0.77 µIU/L, free T4 0.8 ng/dL, free T3 2.8 pg/mL); the patient was euthyroid on methimazole for at least 2 months before surgery. (C) One euthyroid patient with benign thyroid nodules had only subcutaneous adipose tissue (no deep adipose tissue) removed during the surgery. (D) One patient who underwent thyroid surgery for thyroid cancer had a minimally elevated TSH of 4.4 µIU/L before the surgery (reference range 0.36-3.7 µIU/L), not on thyroid hormone treatment; because all prior TSH levels were normal, this patient was included in the euthyroid patient group. (E) One patient with an untreated hyperfunctioning thyroid nodule had thyroid function tests in the subclinical hyperthyroid range for at least 1.5 years before surgery; TSH 0.07 µIU/L (reference range 0.4-4.5 µIU/L), free T4 1.2 ng/dL (reference range 0.8-1.8 ng/dL), free T3 3.9 pg/mL (reference range 2.3-4.2 pg/mL) at surgery time. (F) One patient with Graves’ disease took methimazole in the past for only 1 month and then stopped taking this medication 6 months before thyroid surgery; the patient also did not take Lugol's solution, as prescribed before the surgery. Thyroid function tests showed overt hyperthyroidism at the surgery time (TSH 0.02 µ IU/L [reference range 0.4-4.5 µIU/L], free T4 3.4 ng/dL [reference range 0.93-1.7 ng/dL]).
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    Thyroid status of the study patients at the time of the thyroid surgery. (A) Two patients with Graves’ disease on methimazole who also took Lugol's solution for 10 days before the surgery had thyroid function tests in the overt hypothyroid range at the time of the surgery (TSH 18.17 µ IU/L [reference range 0.4-4.5 µIU/L], free T4 0.6 ng/dL [reference range 0.8-1.8 ng/dL], <t>free</t> <t>T3</t> 3.0 pg/mL [reference range 2.3-4.2 pg/mL]; TSH 18.48 µIU/L, free T4 0.4 ng/dL, free T3 2.2 pg/mL), respectively; 1 patient was hypothyroid for 5 weeks, whereas the second patient was euthyroid/hypothyroid for 4 weeks while taking methimazole before starting Lugol's solution before the thyroid surgery. (B) One patient with a hyperfunctioning thyroid nodule/Graves’ disease who was on methimazole and took Lugol's solution for 10 days before the surgery had thyroid function tests within normal range at the time of the surgery (TSH 0.77 µIU/L, free T4 0.8 ng/dL, free T3 2.8 pg/mL); the patient was euthyroid on methimazole for at least 2 months before surgery. (C) One euthyroid patient with benign thyroid nodules had only subcutaneous adipose tissue (no deep adipose tissue) removed during the surgery. (D) One patient who underwent thyroid surgery for thyroid cancer had a minimally elevated TSH of 4.4 µIU/L before the surgery (reference range 0.36-3.7 µIU/L), not on thyroid hormone treatment; because all prior TSH levels were normal, this patient was included in the euthyroid patient group. (E) One patient with an untreated hyperfunctioning thyroid nodule had thyroid function tests in the subclinical hyperthyroid range for at least 1.5 years before surgery; TSH 0.07 µIU/L (reference range 0.4-4.5 µIU/L), free T4 1.2 ng/dL (reference range 0.8-1.8 ng/dL), free T3 3.9 pg/mL (reference range 2.3-4.2 pg/mL) at surgery time. (F) One patient with Graves’ disease took methimazole in the past for only 1 month and then stopped taking this medication 6 months before thyroid surgery; the patient also did not take Lugol's solution, as prescribed before the surgery. Thyroid function tests showed overt hyperthyroidism at the surgery time (TSH 0.02 µ IU/L [reference range 0.4-4.5 µIU/L], free T4 3.4 ng/dL [reference range 0.93-1.7 ng/dL]).
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    Thyroid status of the study patients at the time of the thyroid surgery. (A) Two patients with Graves’ disease on methimazole who also took Lugol's solution for 10 days before the surgery had thyroid function tests in the overt hypothyroid range at the time of the surgery (TSH 18.17 µ IU/L [reference range 0.4-4.5 µIU/L], free T4 0.6 ng/dL [reference range 0.8-1.8 ng/dL], <t>free</t> <t>T3</t> 3.0 pg/mL [reference range 2.3-4.2 pg/mL]; TSH 18.48 µIU/L, free T4 0.4 ng/dL, free T3 2.2 pg/mL), respectively; 1 patient was hypothyroid for 5 weeks, whereas the second patient was euthyroid/hypothyroid for 4 weeks while taking methimazole before starting Lugol's solution before the thyroid surgery. (B) One patient with a hyperfunctioning thyroid nodule/Graves’ disease who was on methimazole and took Lugol's solution for 10 days before the surgery had thyroid function tests within normal range at the time of the surgery (TSH 0.77 µIU/L, free T4 0.8 ng/dL, free T3 2.8 pg/mL); the patient was euthyroid on methimazole for at least 2 months before surgery. (C) One euthyroid patient with benign thyroid nodules had only subcutaneous adipose tissue (no deep adipose tissue) removed during the surgery. (D) One patient who underwent thyroid surgery for thyroid cancer had a minimally elevated TSH of 4.4 µIU/L before the surgery (reference range 0.36-3.7 µIU/L), not on thyroid hormone treatment; because all prior TSH levels were normal, this patient was included in the euthyroid patient group. (E) One patient with an untreated hyperfunctioning thyroid nodule had thyroid function tests in the subclinical hyperthyroid range for at least 1.5 years before surgery; TSH 0.07 µIU/L (reference range 0.4-4.5 µIU/L), free T4 1.2 ng/dL (reference range 0.8-1.8 ng/dL), free T3 3.9 pg/mL (reference range 2.3-4.2 pg/mL) at surgery time. (F) One patient with Graves’ disease took methimazole in the past for only 1 month and then stopped taking this medication 6 months before thyroid surgery; the patient also did not take Lugol's solution, as prescribed before the surgery. Thyroid function tests showed overt hyperthyroidism at the surgery time (TSH 0.02 µ IU/L [reference range 0.4-4.5 µIU/L], free T4 3.4 ng/dL [reference range 0.93-1.7 ng/dL]).
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    Thyroid status of the study patients at the time of the thyroid surgery. (A) Two patients with Graves’ disease on methimazole who also took Lugol's solution for 10 days before the surgery had thyroid function tests in the overt hypothyroid range at the time of the surgery (TSH 18.17 µ IU/L [reference range 0.4-4.5 µIU/L], free T4 0.6 ng/dL [reference range 0.8-1.8 ng/dL], <t>free</t> <t>T3</t> 3.0 pg/mL [reference range 2.3-4.2 pg/mL]; TSH 18.48 µIU/L, free T4 0.4 ng/dL, free T3 2.2 pg/mL), respectively; 1 patient was hypothyroid for 5 weeks, whereas the second patient was euthyroid/hypothyroid for 4 weeks while taking methimazole before starting Lugol's solution before the thyroid surgery. (B) One patient with a hyperfunctioning thyroid nodule/Graves’ disease who was on methimazole and took Lugol's solution for 10 days before the surgery had thyroid function tests within normal range at the time of the surgery (TSH 0.77 µIU/L, free T4 0.8 ng/dL, free T3 2.8 pg/mL); the patient was euthyroid on methimazole for at least 2 months before surgery. (C) One euthyroid patient with benign thyroid nodules had only subcutaneous adipose tissue (no deep adipose tissue) removed during the surgery. (D) One patient who underwent thyroid surgery for thyroid cancer had a minimally elevated TSH of 4.4 µIU/L before the surgery (reference range 0.36-3.7 µIU/L), not on thyroid hormone treatment; because all prior TSH levels were normal, this patient was included in the euthyroid patient group. (E) One patient with an untreated hyperfunctioning thyroid nodule had thyroid function tests in the subclinical hyperthyroid range for at least 1.5 years before surgery; TSH 0.07 µIU/L (reference range 0.4-4.5 µIU/L), free T4 1.2 ng/dL (reference range 0.8-1.8 ng/dL), free T3 3.9 pg/mL (reference range 2.3-4.2 pg/mL) at surgery time. (F) One patient with Graves’ disease took methimazole in the past for only 1 month and then stopped taking this medication 6 months before thyroid surgery; the patient also did not take Lugol's solution, as prescribed before the surgery. Thyroid function tests showed overt hyperthyroidism at the surgery time (TSH 0.02 µ IU/L [reference range 0.4-4.5 µIU/L], free T4 3.4 ng/dL [reference range 0.93-1.7 ng/dL]).
    Free T3 Ft3, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thyroid status of the study patients at the time of the thyroid surgery. (A) Two patients with Graves’ disease on methimazole who also took Lugol's solution for 10 days before the surgery had thyroid function tests in the overt hypothyroid range at the time of the surgery (TSH 18.17 µ IU/L [reference range 0.4-4.5 µIU/L], free T4 0.6 ng/dL [reference range 0.8-1.8 ng/dL], <t>free</t> <t>T3</t> 3.0 pg/mL [reference range 2.3-4.2 pg/mL]; TSH 18.48 µIU/L, free T4 0.4 ng/dL, free T3 2.2 pg/mL), respectively; 1 patient was hypothyroid for 5 weeks, whereas the second patient was euthyroid/hypothyroid for 4 weeks while taking methimazole before starting Lugol's solution before the thyroid surgery. (B) One patient with a hyperfunctioning thyroid nodule/Graves’ disease who was on methimazole and took Lugol's solution for 10 days before the surgery had thyroid function tests within normal range at the time of the surgery (TSH 0.77 µIU/L, free T4 0.8 ng/dL, free T3 2.8 pg/mL); the patient was euthyroid on methimazole for at least 2 months before surgery. (C) One euthyroid patient with benign thyroid nodules had only subcutaneous adipose tissue (no deep adipose tissue) removed during the surgery. (D) One patient who underwent thyroid surgery for thyroid cancer had a minimally elevated TSH of 4.4 µIU/L before the surgery (reference range 0.36-3.7 µIU/L), not on thyroid hormone treatment; because all prior TSH levels were normal, this patient was included in the euthyroid patient group. (E) One patient with an untreated hyperfunctioning thyroid nodule had thyroid function tests in the subclinical hyperthyroid range for at least 1.5 years before surgery; TSH 0.07 µIU/L (reference range 0.4-4.5 µIU/L), free T4 1.2 ng/dL (reference range 0.8-1.8 ng/dL), free T3 3.9 pg/mL (reference range 2.3-4.2 pg/mL) at surgery time. (F) One patient with Graves’ disease took methimazole in the past for only 1 month and then stopped taking this medication 6 months before thyroid surgery; the patient also did not take Lugol's solution, as prescribed before the surgery. Thyroid function tests showed overt hyperthyroidism at the surgery time (TSH 0.02 µ IU/L [reference range 0.4-4.5 µIU/L], free T4 3.4 ng/dL [reference range 0.93-1.7 ng/dL]).
    Rat Ft3, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thyroid status of the study patients at the time of the thyroid surgery. (A) Two patients with Graves’ disease on methimazole who also took Lugol's solution for 10 days before the surgery had thyroid function tests in the overt hypothyroid range at the time of the surgery (TSH 18.17 µ IU/L [reference range 0.4-4.5 µIU/L], free T4 0.6 ng/dL [reference range 0.8-1.8 ng/dL], free T3 3.0 pg/mL [reference range 2.3-4.2 pg/mL]; TSH 18.48 µIU/L, free T4 0.4 ng/dL, free T3 2.2 pg/mL), respectively; 1 patient was hypothyroid for 5 weeks, whereas the second patient was euthyroid/hypothyroid for 4 weeks while taking methimazole before starting Lugol's solution before the thyroid surgery. (B) One patient with a hyperfunctioning thyroid nodule/Graves’ disease who was on methimazole and took Lugol's solution for 10 days before the surgery had thyroid function tests within normal range at the time of the surgery (TSH 0.77 µIU/L, free T4 0.8 ng/dL, free T3 2.8 pg/mL); the patient was euthyroid on methimazole for at least 2 months before surgery. (C) One euthyroid patient with benign thyroid nodules had only subcutaneous adipose tissue (no deep adipose tissue) removed during the surgery. (D) One patient who underwent thyroid surgery for thyroid cancer had a minimally elevated TSH of 4.4 µIU/L before the surgery (reference range 0.36-3.7 µIU/L), not on thyroid hormone treatment; because all prior TSH levels were normal, this patient was included in the euthyroid patient group. (E) One patient with an untreated hyperfunctioning thyroid nodule had thyroid function tests in the subclinical hyperthyroid range for at least 1.5 years before surgery; TSH 0.07 µIU/L (reference range 0.4-4.5 µIU/L), free T4 1.2 ng/dL (reference range 0.8-1.8 ng/dL), free T3 3.9 pg/mL (reference range 2.3-4.2 pg/mL) at surgery time. (F) One patient with Graves’ disease took methimazole in the past for only 1 month and then stopped taking this medication 6 months before thyroid surgery; the patient also did not take Lugol's solution, as prescribed before the surgery. Thyroid function tests showed overt hyperthyroidism at the surgery time (TSH 0.02 µ IU/L [reference range 0.4-4.5 µIU/L], free T4 3.4 ng/dL [reference range 0.93-1.7 ng/dL]).

    Journal: Journal of the Endocrine Society

    Article Title: Thermogenic Gene Expression in Human Neck Adipose Tissue in Relation to Circulating and Local Thyroid Hormone Levels

    doi: 10.1210/jendso/bvaf178

    Figure Lengend Snippet: Thyroid status of the study patients at the time of the thyroid surgery. (A) Two patients with Graves’ disease on methimazole who also took Lugol's solution for 10 days before the surgery had thyroid function tests in the overt hypothyroid range at the time of the surgery (TSH 18.17 µ IU/L [reference range 0.4-4.5 µIU/L], free T4 0.6 ng/dL [reference range 0.8-1.8 ng/dL], free T3 3.0 pg/mL [reference range 2.3-4.2 pg/mL]; TSH 18.48 µIU/L, free T4 0.4 ng/dL, free T3 2.2 pg/mL), respectively; 1 patient was hypothyroid for 5 weeks, whereas the second patient was euthyroid/hypothyroid for 4 weeks while taking methimazole before starting Lugol's solution before the thyroid surgery. (B) One patient with a hyperfunctioning thyroid nodule/Graves’ disease who was on methimazole and took Lugol's solution for 10 days before the surgery had thyroid function tests within normal range at the time of the surgery (TSH 0.77 µIU/L, free T4 0.8 ng/dL, free T3 2.8 pg/mL); the patient was euthyroid on methimazole for at least 2 months before surgery. (C) One euthyroid patient with benign thyroid nodules had only subcutaneous adipose tissue (no deep adipose tissue) removed during the surgery. (D) One patient who underwent thyroid surgery for thyroid cancer had a minimally elevated TSH of 4.4 µIU/L before the surgery (reference range 0.36-3.7 µIU/L), not on thyroid hormone treatment; because all prior TSH levels were normal, this patient was included in the euthyroid patient group. (E) One patient with an untreated hyperfunctioning thyroid nodule had thyroid function tests in the subclinical hyperthyroid range for at least 1.5 years before surgery; TSH 0.07 µIU/L (reference range 0.4-4.5 µIU/L), free T4 1.2 ng/dL (reference range 0.8-1.8 ng/dL), free T3 3.9 pg/mL (reference range 2.3-4.2 pg/mL) at surgery time. (F) One patient with Graves’ disease took methimazole in the past for only 1 month and then stopped taking this medication 6 months before thyroid surgery; the patient also did not take Lugol's solution, as prescribed before the surgery. Thyroid function tests showed overt hyperthyroidism at the surgery time (TSH 0.02 µ IU/L [reference range 0.4-4.5 µIU/L], free T4 3.4 ng/dL [reference range 0.93-1.7 ng/dL]).

    Article Snippet: Serum thyroid function tests (TFTs): TSH, free T4 (FT4), and free T3 (FT3) were measured preoperatively by chemiluminescent immunoassays in a fasting state for 9 patients (Quest Diagnostics); when not available, outpatient or immediate postoperative labs were used.

    Techniques:

    Correlations between serum TSH, T4, and T3, and local adipose tissue T3 and T4 levels. (A) Serum TSH and serum free T4, (B) Serum TSH and serum free T3, (C) Serum free T4 and serum free T3, (D) Serum TSH and tissue T3, (E) Serum TSH and tissue T4, (F) Serum free T4 and tissue T4, (G) Serum free T3 and tissue T3. A P < .05 is considered statistically significant.

    Journal: Journal of the Endocrine Society

    Article Title: Thermogenic Gene Expression in Human Neck Adipose Tissue in Relation to Circulating and Local Thyroid Hormone Levels

    doi: 10.1210/jendso/bvaf178

    Figure Lengend Snippet: Correlations between serum TSH, T4, and T3, and local adipose tissue T3 and T4 levels. (A) Serum TSH and serum free T4, (B) Serum TSH and serum free T3, (C) Serum free T4 and serum free T3, (D) Serum TSH and tissue T3, (E) Serum TSH and tissue T4, (F) Serum free T4 and tissue T4, (G) Serum free T3 and tissue T3. A P < .05 is considered statistically significant.

    Article Snippet: Serum thyroid function tests (TFTs): TSH, free T4 (FT4), and free T3 (FT3) were measured preoperatively by chemiluminescent immunoassays in a fasting state for 9 patients (Quest Diagnostics); when not available, outpatient or immediate postoperative labs were used.

    Techniques:

    Enriched pathways correlated with serum free T3 concentration. (A) Normalized enrichment score of the top 5 correlated pathways in deep and subcutaneous neck adipose tissue samples. (B) Normalized enrichment score of thermogenic pathways. Fill reflects the sign of the normalized enrichment score (NES). A positive NES indicates pathway upregulation, and a negative NES indicates pathway downregulation. *FDR < 0.05.

    Journal: Journal of the Endocrine Society

    Article Title: Thermogenic Gene Expression in Human Neck Adipose Tissue in Relation to Circulating and Local Thyroid Hormone Levels

    doi: 10.1210/jendso/bvaf178

    Figure Lengend Snippet: Enriched pathways correlated with serum free T3 concentration. (A) Normalized enrichment score of the top 5 correlated pathways in deep and subcutaneous neck adipose tissue samples. (B) Normalized enrichment score of thermogenic pathways. Fill reflects the sign of the normalized enrichment score (NES). A positive NES indicates pathway upregulation, and a negative NES indicates pathway downregulation. *FDR < 0.05.

    Article Snippet: Serum thyroid function tests (TFTs): TSH, free T4 (FT4), and free T3 (FT3) were measured preoperatively by chemiluminescent immunoassays in a fasting state for 9 patients (Quest Diagnostics); when not available, outpatient or immediate postoperative labs were used.

    Techniques: Concentration Assay